LOCUS       pCE-Zero\vector         3957 bp    DNA     circular SYN 16-JAN-2018
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COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     source          319..3957
                     /invitrogen="244"
                     /vntifkey="98"
     CDS             508..810
                     /invitrogen="1070000"
                     /vntifkey="4"
                     /label=ccdB\(no\ATG)
     promoter        1021..1158
                     /invitrogen="2350000"
                     /vntifkey="30"
                     /label=Kan\promoter
     CDS             1159..1953
                     /invitrogen="1200000"
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      3162..3835
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     primer          complement(328..347)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        complement(331..347)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    complement(354..370)
                     /vntifkey="21"
                     /label=M13F
     misc_feature    205..221
                     /vntifkey="21"
                     /label=M13R
     misc_feature    complement(2112..3109)
                     /vntifkey="21"
                     /label=Amp
     misc_feature    289..303
                     /vntifkey="21"
                     /label=URS
     misc_feature    304..318
                     /vntifkey="21"
                     /label=DRS
     misc_feature    217..219
                     /vntifkey="21"
                     /label=ATG
     misc_feature    179..197
                     /vntifkey="21"
                     /label=TSS
     misc_feature    303..304
                     /vntifkey="21"
                     /label=Cloning\Site
BASE COUNT      938 a      1012 c      1039 g       968 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca 
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgg atcttccaga 
      301 gatatcgtcg aacggcagtt gaattcgccc tatagtgagt cgtattacaa ttcactggcc 
      361 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 
      421 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 
      481 caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc 
      541 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg 
      601 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg 
      661 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg 
      721 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac 
      781 gccattaacc tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc 
      841 ttcacctaga tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg 
      901 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta 
      961 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa 
     1021 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg 
     1081 atggctttct cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca 
     1141 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 
     1201 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 
     1261 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 
     1321 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc 
     1381 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 
     1441 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 
     1501 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 
     1561 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 
     1621 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 
     1681 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 
     1741 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 
     1801 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 
     1861 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 
     1921 gccttctatc gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg 
     1981 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacaggt ggcacttttc 
     2041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     2101 cgctcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     2161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     2221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     2281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     2341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     2401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     2461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     2521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     2581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     2641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     2701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     2761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     2821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     2881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     2941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     3001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     3061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgacca 
     3121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     3181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     3241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     3301 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     3361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     3421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     3481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     3541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     3601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     3661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     3721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     3781 ccagcaacgc ggccttttta cggttcctgg gcttttgctg gccttttgct cacatgttct 
     3841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     3901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag 
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