LOCUS       pCE2\TA/Blunt-Ze        3957 bp    DNA     circular SYN  9-NOV-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by VectorDesigner
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|-12546589|
COMMENT     VNTDBDATE|-12546589|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
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FEATURES             Location/Qualifiers
     source          417..3957
                     /invitrogen="243"
                     /vntifkey="98"
     promoter        1143..1280
                     /invitrogen="2350000"
                     /vntifkey="30"
                     /label=Kan\promoter
     CDS             1281..2075
                     /invitrogen="1200000"
                     /vntifkey="4"
                     /label=Kan
     rep_origin      3284..3957
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     protein_bind    complement(417..421)
                     /invitrogen="2460000"
                     /vntifkey="31"
                     /label=TOPO\binding\site
     overhang        complement(417..417)
                     /vntifkey="274"
                     /label=3'-T\overhang
                     /note="Unknown feature type:overhang"
     misc_feature    complement(2226..3239)
                     /vntifkey="21"
                     /label=Amp(R)
     misc_feature    301..319
                     /vntifkey="21"
                     /label=TSS
     misc_feature    327..343
                     /vntifkey="21"
                     /label=M13\reverse\primer
     misc_feature    217..338
                     /vntifkey="21"
                     /label=Lac\promter
     misc_feature    339..932
                     /vntifkey="21"
                     /label=LacZ\ccdB\fragment
     misc_feature    412..416
                     /vntifkey="21"
                     /label=TOPO\binding\site
     misc_feature    476..492
                     /vntifkey="21"
                     /label=M13\forward\primer
BASE COUNT      945 a      1010 c      1034 g       968 t 
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag taagtgagcg aggaagcgga 
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct 
      361 cagaattaac cctcactaaa ggtactagtc ctgcaggttt aaacgaattc gcccttaagg 
      421 gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga atcgtattac aattcactgg 
      481 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
      541 cagcacatcc acctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgcccgt 
      601 cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga 
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga 
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc 
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc 
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa 
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagca 
      961 tcttcaccta gatcgttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga 
     1021 tgaatgtcag ctactgggct atctggacaa gagaaaacgc aagcgcaaag agaaagcagg 
     1081 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg 
     1141 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact 
     1201 ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga 
     1261 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 
     1321 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 
     1381 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 
     1441 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg 
     1501 gcgttccatg cgcagctgtg ctcgacgttg tcactgaagc gggaagagac tggctgctat 
     1561 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 
     1621 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 
     1681 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 
     1741 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 
     1801 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 
     1861 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 
     1921 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 
     1981 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 
     2041 tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga 
     2101 tgcggtattt tctcgttacg catctgtgcg gtatttcaca ccgcatacag gtggcacttt 
     2161 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
     2221 tccgctcatg agattatcaa ataggatctt cacctagatc ctgttaaatt aaaaatgaag 
     2281 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     2341 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactacc 
     2401 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     2461 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     2521 agccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     2581 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     2641 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     2701 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctctttcgg 
     2761 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     2821 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     2881 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     2941 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     3001 ttcttcgggg cgaaaactct caagaatctt accgctgttg agatccagtt cgatgtaacc 
     3061 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     3121 aaaaacagga aggcaaaatg ccgcaaaaaa cggaataaga gcgacacgga aatgttgaat 
     3181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgac 
     3241 caaaatccgt taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     3301 acgatcttct tgagatcgtt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     3361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     3421 aactggcttc agcagagcgc agataccaaa tactgtcgtt ctagtgtagc cgtagttagg 
     3481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     3541 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggtctcaa gacgatagtt 
     3601 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     3661 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     3721 tcccgaagcg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     3781 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     3841 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     3901 cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg ctcacat 
//
